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combinedgenecaller_merge

Description

MGnify merging script for combined gene caller. The merged output contains all the gene predictions from Pyrodigal, along with genes predicted by FragGeneScanRS that do not overlap with any Pyrodigal gene. If mask file is provided, it masks (removes) genes that overlap with regions from a masking file.

Keywords: gene finding, prokaryotes, microbial

Installation

ebi-metagenomics/combinedgenecaller/merge

nf-core modules -g https://www.github.com/ebi-metagenomics/nf-modules install combinedgenecaller/merge

Tools

combinedgenecaller

This script merges the gene prediction with Pyrodigal and FragGeneScanRS

Input

Name Type Description Pattern
meta map Groovy Map containing sample information e.g. [ id:'test', single_end:false ] -
pyrodigal_gff file gene annotations in GFF format produced by Pyrodigal *.gff
pyrodigal_ffn file nucleotide sequences of genes predicted by Pyrodigal *.{ffn,fna}
pyrodigal_faa file protein translations of genes predicted by Pyrodigal *.faa
fgs_gff file gene annotations in GFF format produced by FragGeneScanRS *.gff
fgs_ffn file nucleotide sequences of genes predicted by FragGeneScanRS *.{ffn,fna}
fgs_faa file protein translations of genes predicted by FragGeneScanRS *.faa
mask file sequence mask file (tblout files generated by Infernal cmscan or cmsearch) *.*

Output

Name Type Description Pattern
faa file -
ffn file -
gff file -
versions file -

People

Authors

Maintainers