Add results from MGnifyR to HoloFoodR results
Usage
addMGnify(x, y, ...)
# S4 method for class 'SummarizedExperiment,MultiAssayExperiment'
addMGnify(
x,
y,
exp.name1 = "metagenomic",
exp.name2 = "metagenomic_amplicon",
replace = TRUE,
...
)
# S4 method for class 'SummarizedExperiment,SummarizedExperiment'
addMGnify(x, y, ...)
# S4 method for class 'SummarizedExperiment,ANY'
addMGnify(x, y, id.col1 = "sample_biosample", id.col2 = "accession", ...)
Arguments
- x
SummarizedExperiment
. Results fromMGnifyR::getResult()
.- y
MultiAssayExperiment
orSummarizedExperiment
ordata.frame
-like table. Results fromHoloFoodR::getResult()
or sample metadata from it.- ...
optional arguments not used currently.
- exp.name1
Character scalar
. Specifies the name of experiment that will be added toy
. (Default:"metagenomic"
)- exp.name2
Character scalar
. Specifies the name of experiment from HoloFoodR results. This experiment is used to match IDs with MGnify data. (Default:"metagenomic_amplicon"
)- replace
Logical scalar
. Whether to replace the template experiment. (Default:TRUE
)- id.col1
Character scalar
. Specifies the name of column fromcolData(x)
that includes HoloFood identifiers. (Default:"sample_biosample"
)- id.col2
Character scalar
. Specifies the name of column fromcolData(y[[exp.name2]])
that includes HoloFood identifiers. (Default:"accession"
)
Details
Metagenomic data is found in MGnify rather than HoloFoodR, and the two
databases use different sample identifiers. However, MGnify's sample
metadata includes references to the identifiers used in the HoloFood
database, making it straightforward to convert sample IDs for alignment
with HoloFood data. Despite this, HoloFood contains additional metadata
not available in MGnify. Moreover, integrating data into a
MultiAssayExperiment
while maintaining accurate sample and system
matches can be challenging.
This function is designed to simplify these
tasks, enabling seamless integration of MGnify data with HoloFood data after
retrieval from the database. You need only to input the returned data from
MGnifyR::getResult()
and HoloFoodR::getResult()
functions.
Examples
if (FALSE) { # \dontrun{
# Get data from HoloFood database
mae <- HoloFoodR::getResult(
salmon_sample_ids,
use.cache = TRUE
)
# Get data from MGnify database
mg <- MgnifyClient(
useCache = TRUE,
cacheDir = ".MGnifyR_cache"
)
tse <- MGnifyR::getResult(
mg,
accession = mgnify_analyses_ids,
get.func = FALSE
)
# Add MGnify data to HoloFood data
mae <- addMGnify(tse, mae)
} # }